投稿分類 其他

主委發表種類: 口頭

投稿標題(中): 探討MRSA對Chlohexidine的抗藥性

投稿標題(英): Chlorhexidine resistance in Methicillin resistant Staphylococcus aureus

投稿摘要: Objectives：Chlorhexidine is a common antimicrobial agent. Chlorhexidine is effective on both Gram-positive and Gram-negative bacteria, although it is less effective with some Gram-negative bacteria. According to the previous studies, no difference was demonstrable between tincture of chlorhexidine and povidone-iodine when used for cutaneous antisepsis. We found that a relatively high prevalence of CA-MRSA colonization in our patient population. This study sought to determine if the use of chlorhexidine could eradicate the colonization of CA-MRSA in our patients. Methods：From February to August of 2013, 80 swabs were obtained from 80 different patients. All specimens were cultured on oxacillin resistance screening agar base. Rapid detection of methicillin resistance in Staphylococcus aureus isolates by the MRSA-screen latex agglutination test were then performed.
Result： In our 80 specimens, CA-MRSA was isolated from 46 cases. Then We performed chlorhexidine MIC on the MRSA isolates. The results showed that the MIC values of chlorhexidine for 3, 16 and 20 MRSA isolates were 1, 2 and 4 µg/ml respectively. MIC breakpoints were defined as susceptible, ≤4 μg/mL. The correlation between the existence of the chlorhexidine-resistant genes qacA and qacB (qacA/B) in methicillin-resistant Staphylococcus aureus (MRSA) was confirmed by the previous study. After we analysis our MRSA isolates, we found that 20 MRSA isolates harbored qacA/B. The MIC values of chlorhexidine for 4, 10 and 6 MRSA isolates which harbored qacA/B were 2, 4 and 8 µg/ml respectively.Discussion：We concluded that the MRSA isolates were resistant to chlorhexidine. The chlorhexidine resistance loci qacA/B correlated to the in vitro study.

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